Author/Authors :
Hamidinia، Maryam نويسنده Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. , , Ghafourian Boroujerdnia، Mehri نويسنده Immunology Department, Medical College, Ahvaz Jundishapur University of Medical Science, Ahvaz, IR Iran Ghafourian Boroujerdnia, Mehri , Talaiezadeh، Abdolhassan نويسنده Research Institute for Infectious Diseases of Digestive System, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran Talaiezadeh, Abdolhassan , Solgi، Ghasem نويسنده Immunology Department, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. , , Roshani، Reyhaneh نويسنده Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. , , Iranprast، Sara نويسنده Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. , , Khodadadi، Abbas ali نويسنده ,
Abstract :
Purpose: Currently, cancer as a major problem around the world threatens human health
and has a high incidence in developing countries. Many reports have indicated that patients
suffering from cancer demonstrate decreased antitumor immune responses as well as a high
prevalence of T regulatory population. It has been reported that Foxp3+Tregs exert
suppression by cell contact-dependent mechanisms which are mediated by soluble factors
such as immunosuppressive cytokines like IL-10, TGF-B and IL-35. Consequently there is a
great need to identify prognostic and diagnostic biomarkers of regulatory T cells for vaccine
and drug development.
Methods: In this study IL-10, TGF-B, IL-35 and Foxp3 mRNA gene expression has been
measured in peripheral blood of 40 breast cancer patients and 40 normal age-matched
women using quantitative real-time PCR (qRT-PCR) method with Master Mix reaction
containing SYBER Green. GAPDH gene was used as housekeeping gene.
Results: Our data demonstrated a significant up-regulation of IL-10, TGF-B, P35, EBI3 and
Foxp3 gene expression in patients’ peripheral blood compared to normal healthy controls
(p < 0.05).
Conclusion: The data suggests that the immune system is suppressed in breast cancer
patients, which may be due to elevated Treg cells population. These results may be useful for
diagnostic or therapeutic purposes. However it may require more investigations
