Title of article :
Involvement of intracellular oxidative stress-sensitive pathway in phloxine B-induced photocytotoxicity in human T lymphocytic leukemia cells
Author/Authors :
Qi ، نويسنده , , Hang and Zhu، نويسنده , , Beiwei and Abe، نويسنده , , Naomi and Shin، نويسنده , , Yuko and Murata، نويسنده , , Yoshiyuki and Nakamura، نويسنده , , Yoshimasa، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Pages :
7
From page :
1841
To page :
1847
Abstract :
We investigated the molecular mechanisms underlying phloxine B (PhB)-induced photocytotoxicity in human T lymphocytic leukemia Jurkat cells. In addition to apoptosis-related biochemical events, photo-irradiated PhB generated intracellular reactive oxygen species (ROS), induced phosphorylation of c-Jun-N-terminal kinase (JNK) in an oxidative stress-dependent manner and up-regulated the gene expression of interferon (IFN)-γ, an inducer of diverse apoptosis-related molecules in activated T cells. PhB-induced apoptosis was significantly inhibited by N-acetyl-l-cysteine, but not by catalase, indicating that ROS generation occurred intracellularly, and by SP600125 and AG490, specific inhibitors of JNK and IFN-γ signaling, respectively, confirming their roles in the apoptotic pathway. IFN-γ up-regulation was also inhibited by SP600125, indicating that it was downstream of JNK activation. These results suggest that PhB-induced apoptosis in Jurkat cells partially involves the intracellular oxidative stress-sensitive and T cell-specific IFN-γ pathway. These data present a novel insight into the mechanisms of photocytotoxicity induced by artificial food colorants in human T lymphocytic leukemia cells.
Keywords :
Jurkat cells , Phloxine B , Photocytotoxicity , JNK , IFN-?
Journal title :
Food and Chemical Toxicology
Serial Year :
2012
Journal title :
Food and Chemical Toxicology
Record number :
2123555
Link To Document :
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