Author/Authors :
Jafari، Mohsen نويسنده Pediatric Infections Research Center, Department of Pediatric Infectious Diseases, Shahid Beheshti University of Medical Sciences , , Fallah، Fatemeh نويسنده , , Borhan، Rebwar Shams نويسنده Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran , , Navidinia، Masoumeh نويسنده Department of Medical Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran , , Karimi، Abdollah نويسنده , , Rafiei Tabatabaei، Sedigheh نويسنده Pediatric Infections Research Center, Department of Pediatric Infectious Diseases, Shahid Beheshti University of Medical Sciences , , Hashemi، Ali نويسنده Shahid Beheshti University of Medical Sciences ,
Abstract :
Serious infections by Pseudomonas aeruginosa are commonly treated with the combination of a beta-lactam antibacterial and an aminoglycoside. Therefore, production of a 16S rRNA methylase may result in an extremely important antibacterial resistance profile. The present study was conducted to determine the prevalence of Cephamycinase (CMY), aminoglycoside 6ʹ-N-acetyltransferase (aac(6′)-Ib) and 16S rRNA methylase genes among Pseudomonas aeruginosa isolates from Iran. This descriptive study was performed on hospitalized burnt patients during 2011and 2012. Antibiotics susceptibility tests were performed by disc diffusion and broth microdilution methods. CMY, aac(6′)-Ib, 16S rRNA methylase genes were detected by PCR method. Seventy-seven (77%) of 100 isolates were resistant to Imipenem and Ceftazidime. aac(6)-Ib, Cephamycinase (CMY), and rRNA methyltransferase (rmtB and rmtD) were detected in 57 (74.02%), 7 (9.09%), 11 (14.28%), and 9 (11.68%) isolates, respectively. PCR results for aminoglycoside resistance methyltransferase (armA) and rRNA methyltransferase (rmtC) were all negative. Aminoglycoside resistance methyltransferase (armA), and rRNA methyltransferase (rmtC) were not detected. This study detected multiple drug resistance in Pseudomonas aeruginosa including resistance to β-lactams, Aminoglycosides, and Fluoroquinolones. Therefore, identification of drug resistance patterns in P. aeruginosa and detection of pan-resistant producing isolates are of great importance in prevention and control of infections in burn center ward.
