Title of article
UV-HPLC / APCI-MS Method for Separation and Identification of the Carotenoids Produced by Sporobolomyces ruberrimus H110
Author/Authors
-، - نويسنده Department of Food Science & Engineering, Faculty of Biosystem Engineering, University of Tehran, Karaj, I.R. IRAN Razavi, Sayed Hadi , -، - نويسنده Laboratoire des Science du Génie Chimique, U.P.R. 6811-C.N.R.S. / I.N.P.L., 13 rue du Bois de la Champelle, 54500 Vandœuvre-Lès-Nancy, FRANCE Blanchard, Fabrice , -، - نويسنده Laboratoire des Science du Génie Chimique, U.P.R. 6811-C.N.R.S. / I.N.P.L., 13 rue du Bois de la Champelle, 54500 Vandœuvre-Lès-Nancy, FRANCE Marc, Ivan
Issue Information
فصلنامه با شماره پیاپی 38 سال 2006
Pages
10
From page
1
To page
10
Abstract
-
Abstract
An on-line UV-HPLC / APCI-MS (atmospheric pressure chemical ionization) tandem by a platinium C18 column with an isocratic solvent system after optimizing was applied to the separation and the identification of different carotenoids including astaxanthin, canthaxanthin, apocarotenoic ester, torularhodin and beta carotene. This method was used to identify particular carotenoids produced by newly isolated microorganism strain of Sporobolomyces ruberrimus H110 in our laboratory. The developed method allows to distinguish torularhodin and canthaxanthin, having the same molar mass but a different chemical structure. A detection limit of 9ng /ml for torularhodin observed. CV (%) for measurement of all carotenoid concentration was always below 3 %. Torularhodin and for a smaller part beta carotene were the two pigments identified in Sporobolmyces ruberrimus H110.
Journal title
Iranian Journal of Chemistry and Chemical Engineering (IJCCE)
Serial Year
2006
Journal title
Iranian Journal of Chemistry and Chemical Engineering (IJCCE)
Record number
2149518
Link To Document