Title of article
Separation of Biojenic Amines Using Dansyl Cloride Derivatization and Mixed Micellar Liquid Chromatography
Author/Authors
-، - نويسنده Department of Chemistry, University of Mazandaran, P.O. Box 453, Babolsar, I.R. IRAN Hadjmohammadi, Mohammad Reza , -، - نويسنده Department of Chemistry, University of Mazandaran, P.O. Box 453, Babolsar, I.R. IRAN Jafari, Soghra
Issue Information
فصلنامه با شماره پیاپی 37 سال 2006
Pages
5
From page
19
To page
23
Abstract
-
Abstract
Separation of some biogenic amines via RP-HPLC using mixed micellar mobile phase was investigated. The compounds were derivatized before hand by dansyl chloride as a chromophoric reagent. Appropriate conditions for separation, were determined by studying factors such as temperature, type and percentage of organic modifier, concentration of surfactants (SDS and Brij-35) and the pH of the mobile phase. The number of theoretical plates (N), asymmetry factor (B/A) and selectivity factor (α) considered as criteria for finding the appropriate conditions for separation. Appropriate condition, were: 40˚C, 40 mM SDS, 0.5 mM Brij-35 and 10% 1-propanol at pH=5.
Journal title
Iranian Journal of Chemistry and Chemical Engineering (IJCCE)
Serial Year
2006
Journal title
Iranian Journal of Chemistry and Chemical Engineering (IJCCE)
Record number
2151832
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