Author/Authors :
Roshangar، Leila نويسنده , , Soleimani-Rad، Jafar نويسنده Drug Applied Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran , , Rashedee، Bahman نويسنده Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran , , Mazochian، Hossein نويسنده Medical Education Research Center, Tabriz University of Medical Sciences, Tabriz, Iran , , Nikzad، Behzad نويسنده Department of Neuroscience, Tabriz University, Tabriz, Iran , , Soleimani Rad، Sara نويسنده Department of Obstetrics and Gynecology, Alzahra Hospital, Faculty of Medicine,Tabriz University of Medical Sciences, Tabriz, Iran ,
Abstract :
Background: Endometrial development has an important role in blastocyst adhesion and implantation. During IVF cycles, endometrial development is enhanced by progesterone. Objective: The aim of this study was to compare ultrastructural and morphometrical characteristics of mice uterine endometrium in natural cycle with those in superovulated cycles received progesterone or Sildenafil. Materials and Methods: In This study, 60 female bulb/c mice were divided into 4 groups: a control and 3 experimental; gonadotropin, gonadotropin+ Sildenafil and gonadotropin+ progesterone. In experimental groups the mice superovulated mated. In the gonadotropin+ progesterone and gonadotropin+ Viagra groups, the mice respectively received 1mg progesterone and 3 mg Sildenafil citrate. Their uterine specimens were prepared for morphometrical and ultrastructural study. Height of the epithelial cells was measured, using motic software. Statistical analysis was performed using ANOVA. Results: Microscopy revealed that in control group the cells had numerous apical microvilli and the height of the cells was 20.52±2.43 ?m. In gonadotropin+ progesterone group, the granules were found in basal and apical portions and cellular height were 17.91±2.78 ?m which were significantly shorter than in the control and gonadotropin groups (p < 0.001). In this group, the apical membrane also contained pinopodes. In gonadotropin +Sildenafil group, the granules were found in both apical and basal portions and the height of the cells were 17.60±2.49 ?m which were significantly shorter than in the control and gonadotropin groups (p < 0.001). In this group, pinopodes appeared slightly extensive than the other groups. Conclusion: It is concluded that superovulatory drugs in mice stimulate endometrial maturation but injection of Sildenafil is nearly more positive.
