Author/Authors :
Palanisamy، Nandhini نويسنده 1Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu, India , , Ramya، Jayaprakash نويسنده 1Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu, India , , Kumar، Srilakshman نويسنده 1Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu, India , , Vasanthi، NS نويسنده 1Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu, India , , Chandran، Preethy نويسنده School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, India , , Khan، Sudheer نويسنده 1Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu, India 2CeNTAB, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamil Nadu, India ,
Abstract :
Petroleum based products are the major source of energy for industries and daily life. Leaks and accidental spills
occur regularly during the exploration, production, refining, transport, and storage of petroleum and petroleum
products. In the present study we isolated the bacteria from diesel contaminated soil and screened them for
diesel biodegradation capacity. One monoculture isolate identified by 16S rRNA gene sequence analysis to be
Acinetobacter baumannii was further studied for diesel oil biodegradation. The effects of various culture parameters
(pH, temperature, NaCl concentrations, initial hydrocarbon concentration, initial inoculum size, role of chemical
surfactant, and role of carbon and nitrogen sources) on biodegradation of diesel oil were evaluated. Optimal diesel
oil biodegradation by A. baumanii occurred at initial pH 7, 35°C and initial hydrocarbon concentration at 4%. The
biodegradation products under optimal cultural conditions were analyzed by GC-MS. The present study suggests
that A. baumannii can be used for effective degradation of diesel oil from industrial effluents contaminated with
diesel oil.
