Author/Authors :
Rezaei Kalantary، Roshanak نويسنده Department of Environmental Health, School of Health, Tehran University of Medical Sciences, Tehran, Iran. Rezaei Kalantary, Roshanak , Mohseni-Bandpi، Anoushiravan نويسنده Department of Environmental Health Engineering, School of Public Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Esrafili، Ali نويسنده Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , , Nasseri، Simin نويسنده 1Department of Environmental Health Engineering, School of Public Health and Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran , , Rashid Ashmagh، Fatemeh نويسنده Department of Environmental Health Engineering, School of Public Health, Iran University of Medical Sciences, Tehran, Iran , , Jorfi، Sahand نويسنده Ph.D student of Environmental health Engineering, Tarbiat Modares University, Tehran, Iran. Jorfi, Sahand , Ja’fari، Mahsa نويسنده Petroleum University of technology, Abadan faculty of petroleum engineering, Abadan, Iran ,
Abstract :
Bioremediation has shown its applicability for removal of polycyclic aromatic hydrocarbons (PAHs) from soil and
sediments. In the present study, the effect of biostimulation on phenanthrene removal from contaminated soil via
adding macro and/or micronutrients and trace elements was investigated. For these purposes three macro nutrients
(as N, P and K), eight micronutrients (as Mg, S, Fe, Cl, Zn, Mn, Cu and Na) and four trace elements (as B, Mo, Co and Ni)
in 11 mineral salts (MS) as variables were used. Placket-Burman statistical design was used to evaluate significance of
variables (MS) in two levels of high and low. A consortium of adapted microorganisms with PAHs was used for
inoculation to the soil slurry which was spiked with phenanthrene in concentration of 500 mg/kg soil. The optimal
reduction resulted when a high level of macro nutrient in the range of 67-87% and low level of micro nutrient in the
range of 12-32% were used with the nitrogen as the dominant macronutrient. The Pareto chart showed that NH4NO3
was the most effective variable in this experiment. The effect of elements on phenanthrene biodegradation showed
following sequence as N > K > P > Cl > Na > Mg. Effectiveness of the other elements in all runs was less than 1%. The
type and concentration of nutrient can play an important role in biodegradation of phenanthrene. Biostimulation with
suitable combination of nutrient can enhance bioremediation of PAHs contaminated soils.
