Author/Authors :
Sahasrabudhe، Madhuri M نويسنده 1Department of Microbiology, Maulana Azad College, Aurangabad, MS, India , , Saratale، Rijuta G نويسنده Department of Biotechnology, Shivaji University, Kolhapur, MS, India , , Saratale، Ganesh D نويسنده 3Department of Environmental Biotechnology, Shivaji University, Kolhapur, MS, IndiaDepartment of Biochemistry, Shivaji University, Kolhapur, MS, India , , Pathade، Girish نويسنده Department of Biotechnology, Fergusson College, Pune, Maharashtra, India ,
Abstract :
Isolated Enterococcus faecalis YZ 66 strain shows ability to decolorize various industrial dyes among which, it showed
complete decolorization and degradation of toxic, sulfonated recalcitrant diazo dye Direct Red 81 (50 mg/L) within
1.5 h of incubation under static anoxic condition. The optimum pH and temperature for decolorization was 7.0 and
40°C, respectively. Significant induction in the activity of intracellular oxidoreductive enzymes suggested its involvement
in the decolorization of Direct Red 81. The biodegradation of Direct Red 81 was monitored by UV-Visible, FT-IR
spectroscopy and HPLC. The final products were characterized by GC-MS and possible pathway of the degradation
of the dye was proposed. The phytotoxicity assay (with respect to plants Sorghum vulgare and Phaseolus mungo)
revealed that the degradation of Direct Red 81 produced nontoxic metabolites. Finally E. faecalis was employed to
decolorize actual industrial effluent showing decolorization (in terms of ADMI value) with moderate COD and BOD
reduction. Moreover the result increases the applicability of the strain for the treatment of industrial wastewaters
containing dye pollutants.
