Author/Authors :
Dom??nguez، نويسنده , , J and Ezquerra، نويسنده , , A and Alonso، نويسنده , , F and McCullough، نويسنده , , K and Summerfield، نويسنده , , A and Bianchi، نويسنده , , A and Zwart، نويسنده , , R.J and Kim، نويسنده , , Y.B and Blecha، نويسنده , , F and Eicher، نويسنده , , S and Murtaugh، نويسنده , , M and Pampusch، نويسنده , , M and Burger، نويسنده , , K، نويسنده ,
Abstract :
Forty five mAbs submitted to the Second International Swine CD workshop were analyzed by six different laboratories for their possible reactivity with porcine myelomonocytic cells using flow cytometry and immunohistochemistry. As a result of these analyses, a new swine workshop cluster, SWC9, composed of two mAbs that recognize an antigen selectively expressed on mature macrophages, was defined. In addition, several mAbs were identified, allowing the differentiation of granulocytes from monocytes/macrophages, or monocytes from macrophages. Further work is required to identify the antigen recognized by these mAbs. Nevertheless, they should already prove useful for the identification of different stages in the macrophage maturation/differentiation, and will certainly aid analyses on the complexity of the mononuclear phagocyte system in the pig. Finally, the cross-reactivity of three anti-human CD14 mAbs with porcine myelomonocytic cells was established in this workshop.
Keywords :
flow cytometry , CD14 mAbs , immunohistochemistry , Porcine myelomonocytic markers
