Title of article :
Characterization and cloning of a major high molecular weight house dust mite allergen (Der f 15) for dogs
Author/Authors :
McCall، نويسنده , , C. and Hunter، نويسنده , , Bradley S. and Stedman، نويسنده , , K. and Weber، نويسنده , , Christine E. and Hillier، نويسنده , , A. and Bozic، نويسنده , , C. and Rivoire، نويسنده , , B. and Olivry، نويسنده , , T.، نويسنده ,
Issue Information :
سالنامه با شماره پیاپی سال 2001
Pages :
17
From page :
231
To page :
247
Abstract :
Although house dust mites (HDM(s)) are important elicitors of canine allergy, the low molecular weight molecules defined as major allergens for humans do not appear to be major allergens for dogs. Western blotting of Dermatophagoides farinae (D. farinae) extracts with sera from sensitized dogs showed that the majority of animals had IgE antibodies specific for two proteins of apparent molecular weights of 98 and 109 kDa (98/109 kDa). The N-terminal sequences of these two proteins were identical, suggesting they were very closely related, and sequencing of internal peptides showed the protein(s) to have homology with insect chitinases. A purified preparation of 98/109 kDa proteins elicited positive intradermal skin tests (IDST(s)) in a group of well-characterized atopic dogs sensitized to D. farinae, but not in normal dogs. A rabbit polyclonal antiserum raised against the purified proteins was used to immunoscreen a D. farinae cDNA library. The mature coding region of the isolated chitinase cDNA predicts a protein of 63.2 kDa; sequence analysis and glycan detection blotting suggest that the molecule is extensively O-glycosylated. Monoclonal antibodies made against the purified native protein were used to localize the chitinase in sections of whole D. farinae mites. The protein displayed an intracellular distribution in the proventriculus and intestine of the mite, suggesting that it has a digestive, rather than a moulting-related, function. The high prevalence of IgE antibodies to this antigen in canine atopic dermatitis makes it a major HDM allergen for dogs, and the protein has been formally designated Der f 15.
Keywords :
atopic dermatitis , Mite allergen , dog , allergen , IGE
Journal title :
Veterinary Immunology and Immunopathology
Serial Year :
2001
Journal title :
Veterinary Immunology and Immunopathology
Record number :
2161516
Link To Document :
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