Title of article :
Quantitative analysis of Fas and Fas ligand mRNAs in a feline T-lymphoid cell line after infection with feline immunodeficiency virus and primary peripheral blood mononuclear cells obtained from cats infected with the virus
Author/Authors :
Mizuno، نويسنده , , T and Goto، نويسنده , , Y and Baba، نويسنده , , K and Momoi، نويسنده , , Y and Endo، نويسنده , , Y and Nishimura، نويسنده , , Y and Masuda، نويسنده , , K and Ohno، نويسنده , , K and Tsujimoto، نويسنده , , H، نويسنده ,
Issue Information :
سالنامه با شماره پیاپی سال 2003
Abstract :
Apoptosis is frequently observed in feline lymphocytes in association with feline immunodeficiency virus (FIV) infection. In this study, to investigate the mechanism of FIV-induced apoptosis, levels of Fas and Fas ligand mRNAs were measured by real-time reverse transcription-PCR. In a feline T-lymphoid cell line the amounts of Fas ligand mRNA increased along with the induction of apoptosis after in vitro infection with FIV. In PBMC collected from 10 cats naturally infected with FIV, Fas ligand mRNA levels were significantly higher than those in PBMC from five uninfected cats. These results indicate that the increased expression of Fas ligand may be involved in the induction of apoptosis of lymphocytes in FIV infection.
Keywords :
Fas , apoptosis , Fas ligand , FIV infection , Real-Time PCR
Journal title :
Veterinary Immunology and Immunopathology
Journal title :
Veterinary Immunology and Immunopathology