Quantitation of bovine macrophage colony-stimulating factor in bovine serum by ELISA

We established an enzyme-linked immunosorbent assay (ELISA) system for the quantitation of bovine macrophage colony-stimulating factor (M-CSF) and used it to measure the serum M-CSF levels in bovine fetuses and calves. The average serum M-CSF level was 2.7±1.5 ng/ml in 39 calves under 100 days old, and 1.8±0.8 ng/ml in 15 cattle between 101 and 418 days old. Fetal sera samples (n=6) prepared from cattle between 150 and 280 days of gestational age had a higher average level of M-CSF (8.8±1.4 ng/ml). Alteration in serum M-CSF levels in each individual calf was also measured. The serum levels of M-CSF in calves at 0–1 day after birth ranged from 0.52 to 7.3 ng/ml. During the period 113–125 days after birth, serum levels were around 1.4±0.39 ng/ml. Although serum M-CSF levels generally decreased as the age of calves advanced, differences among individuals, especially among newborn calves, were observed.