Author/Authors :
Leal، نويسنده , , Alexandre T. and Seixas، نويسنده , , Adriana and Pohl، نويسنده , , Paula C. and Ferreira، نويسنده , , Carlos A.S. and Logullo، نويسنده , , Carlos and Oliveira، نويسنده , , Pedro L. and Farias، نويسنده , , Sandra E. and Termignoni، نويسنده , , Carlos and da Silva Vaz Jr.، نويسنده , , Itabajara and Masuda، نويسنده , , Aoi، نويسنده ,
Abstract :
Boophilus Yolk pro-Cathepsin (BYC) is an aspartic proteinase found in Boophilus microplus eggs that is involved in the embryogenesis and has been tested as antigen to compose an anti-tick vaccine. The vaccine potential of a recombinant BYC expressed in Escherichia coli (rBYC) was investigated. rBYC was purified and used to immunize Hereford cattle. The sera of bovines immunized with rBYC recognized the native BYC with a titer ranging from 125 to 4000. Furthermore, immunized bovines challenged with 20,000 larvae presented an overall protection of 25.24%. The partial protection obtained against B. microplus infestation with the recombinant protein immunization was similar to the already described for native BYC immunization.
Keywords :
BYC , aspartic proteinase , Bovine , cytokine , Boophilus microplus , Tick vaccine
