Quantitative analysis of inflammatory cytokines expression in peripheral blood mononuclear cells of the ferret (Mustela putorius furo) using real-time PCR

This study describes the expression pattern of cytokines, interferon-gamma (IFN-γ), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and IL-10, produced by LPS stimulation in peripheral blood mononuclear cells (PBMCs) of the ferret (Mustela putorius furo). Real-time PCR was used with TaqMan probes, which were modified by dual-labeled probes (TAMRA/FAM), quantitative analysis of cytokine mRNA comparing the cytokine with the housekeeping gene, ferret GAPDH, as the relative Ct value. Expression peaks of IFN-γ, TNF-α, IL-6 mRNA occurred 2 h after LPS stimulation, whereas the IL-10 peak was 8 h post-LPS. In the present study, peak cytokine expression was detected within 8 h, similar to several other mammalian studies. This current study provides baseline information on inflammatory cytokines of ferret PBMCs.