Author/Authors :
Wauters، نويسنده , , Jella and Franck، نويسنده , , Thierry and Pille، نويسنده , , Frederik and Martens، نويسنده , , Ann and Demeyere، نويسنده , , Kristel and Sys، نويسنده , , Stanislas and Serteyn، نويسنده , , Didier and Gasthuys، نويسنده , , Frank and Meyer، نويسنده , , Evelyne، نويسنده ,
Abstract :
Myeloperoxidase (MPO) is a protein of interest due to its involvement in equine pathologies. Until now, results in equine diagnostic research were achieved through extracellular MPO detection. However, studying the cellular MPO content in neutrophils has revealed important insights in human diseases. This study aimed to develop a technique for the specific detection of MPO on the single cell level defining a flow cytometric protocol for the detection of both equine surface-bound and cellular MPO. Both indirect and direct labeling techniques are described which include the comparison of two secondary antibodies and two linking-fluorochromes, respectively.
Keywords :
Horse , Cellular , surface , Myeloperoxidase , neutrophils , flow cytometry
