Author/Authors :
Kubota، نويسنده , , Shota and Miyaji، نويسنده , , Kazuki and Shimo، نويسنده , , Yusaku and Shimakura، نويسنده , , Hidekatsu and Takase، نويسنده , , Yukari and Okamoto، نويسنده , , Noriaki and Kiuchi، نويسنده , , Akio and Fujimura، نويسنده , , Masato and Fujimura، نويسنده , , Takashi and DeBoer، نويسنده , , Douglas J. and Tsukui، نويسنده , , Toshihiro and Sakaguchi، نويسنده , , Masahiro، نويسنده ,
Abstract :
The present study investigated IgE reactivity to a new Cryptomeria japonica pollen allergen (Cry j 3) in dogs with atopic dermatitis by using a fluorometric ELISA. Serum samples from 15 dogs that showed IgE sensitivity to crude C. japonica pollen allergen by ELISA were tested for specific IgE to each allergen, individually. All 15 dogs had anti-Cry j 1 IgE, 6 (40%) had anti-Cry j 2 IgE, and 11 (73%) had anti-Cry j 3 IgE. Further, we found that these anti-Cry j 3 IgE reacted to Cry j 3 with immunoblotting analysis. These findings indicate that Cry j 3 may be a major allergen in dogs.
Keywords :
canine atopic dermatitis , Japanese cedar pollen , IGE , allergen
