Comparative Study on Total Lipid Determination using Soxhlet, Roese-Gottlieb, Bligh & Dyer, and Modified Bligh & Dyer Extraction Methods

Soxhlet method was used in hot and standard extraction mode and compared with liquid–liquid extraction methods for lipid determination in food samples. Several solvent mixtures were investigated and Soxhlet extraction time was optimized. Soxhlet extraction was convenient only for solid samples. Except Roese–Gottlieb method, other liquid–liquid extraction methods were suitable for lipid extraction from all samples analysed. Even if the precision of each method was good (R.S.D.<3.5%), the comparison of extracted lipid amounts showed that, depending on the type of sample, some methods present serious limitations.