Title of article :
Comparative possession of Shiga toxin, intimin, enterohaemolysin and major extended spectrum beta lactamase (ESBL) genes in Escherichia coli isolated from backyard and farmed poultry
Author/Authors :
سامانتا، ايندرانيل نويسنده Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, 37, K. B. Sarani, Kolkata-700037, West Bengal, India Samanta, I , جوآردار، سيدهارتا نارايان نويسنده Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, 37, K. B. Sarani, Kolkata-700037, West Bengal, India Joardar, S. N , داس، پراديپ كومار نويسنده Department of Veterinary Physiology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, 37, K. B. Sarani, Kolkata-700037, West Bengal, India Das, P. K , سار، تاپاس كومار نويسنده Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, 37, K. B. Sarani, Kolkata-700037, West Bengal, India Sar, T. K
Abstract :
مطالعه حاضر براي مقايسه وقوع اشريشيا کولاي داراي ژن‌هاي حدت و ESBL در طيور خانگي و صنعتي انجام گرفت. 360 نمونه از طيور نگهداري شده با سيستم خانگي و 120 نمونه از طيور صنعتي از بنگال غربي و هند جمع‌آوري شدند. از ميان جدايه‌هاي اشريشيا کولاي طيور خانگي O2)، O10، O25، O55، O60 و (UT، هيچ يک از آن‌ها ژن شيگا توکسين را نداشتند و هشت جدايه اشريشيا کولاي (272/8؛ 9/2%) تنها ژن eaeA را داشتند. در حالي که از ميان اشريشيا کولاي جدا شده از طيور صنعتي O17)، O20، O22، O102، O114، O119، rough و (UT، 4 جدايه (48/4؛ 1/5%) ژن stx1/stx2 و 11 جدايه (48/11؛ 1/14%) ژن eaeA را داشتند. هيچ يک از جدايه‌هاي اشريشيا کولاي در طيور خانگي ژن ESBL مورد مطالعه را نداشتند. در حالي که، 4/29% از جدايه‌هاي اشريشيا کولاي در طيور صنعتي ژن ESBL را داشتند.
Abstract :
The present work was conducted to compare the occurrence of Escherichia coli possessing virulence and ESBL genes in backyard and farmed poultry. Three hundred and sixty samples from the poultry kept in backyard system and 120 samples from the farmed birds were collected from West Bengal, India. Among the E. coli isolates of backyard poultry (O2, O10, O25, O55, O60, O106, UT), none of them possessed any of the Shiga toxin genes and eight E. coli isolates (8/272; 2.9%) harboured eaeA gene alone. Whereas among the E. coli isolated from the farmed poultry (O17, O20, O22, O102, O114, O119, rough, UT), four isolates (4/78, 5.1%) harboured stx1/stx2 gene and 11 isolates (11/78, 14.1%) possessed eaeA gene. None of the E. coli isolates from the backyard poultry harboured any studied ESBL gene. Whereas 29.4% of E. coli isolates from the farmed poultry were found to possess the ESBL genes.
