Author/Authors :
Sevilla، نويسنده , , Myrna and Meletzus، نويسنده , , Dietmar and Teixeira، نويسنده , , Katia and Lee، نويسنده , , Sunhee and Nutakki، نويسنده , , Anu and Baldani، نويسنده , , Ivo and Kennedy، نويسنده , , Christina، نويسنده ,
Abstract :
The focus of our collaborative research program is the identification and characterization of nif and related regulatory genes of Acetobacter diazotrophicus in order to understand what factors influence nitrogen fixation in this unique diazotroph. To date, the following genes have been isolated from genomic libraries and are being analyzed: nifHDK, nifA, nifB, nifV, nifE and ntrBC. In addition, Nif− mutants have been constructed by insertional mutagenesis. These mutants are currently being used in inoculation experiments of sterile sugarcane plants to determine whether nitrogen fixed by A. diazotrophicus is significant for plant nutrition.