Author/Authors :
Asadgol، Zahra نويسنده Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155–6451, Tehran 14176, Iran Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , , Forootanfar، Hamid نويسنده 1Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran , Iran,Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, , , Rezaei، Shahla نويسنده 1Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran 14176, Iran , , Mahvi، Amir Hossein نويسنده Department of Environmental Health Engineering, School of Health; National Institute of Health Research; Center for Solid Waste Research, Institute fo , , Faramarzi، Mohammad Ali نويسنده ,
Abstract :
Background: Elimination of hazardous phenolic compounds using laccases has gained attention during recent
decades. The present study was designed to evaluate the ability of the purified laccase from Paraconiothyrium
variabile (PvL) for elimination of phenol and the endocrine disrupting chemical bisphenol A. Effect of laccase
activity, pH, and temperature on the enzymatic removal of the mentioned pollutants were also investigated.
Results: After 30 min treatment of the applied phenolic pollutants in the presence of PvL (5 U/mL), 80% of phenol
and 59.7% of bisphenol A was removed. Increasing of laccase activity enhanced the removal percentage of both
pollutants. The acidic pH of 5 was found to be the best pH for elimination of both phenol and bisphenol A.
Increasing of reaction temperature up to 50°C enhanced the removal percentage of phenol and bisphenol A to
96.3% and 88.3%, respectively.
Conclusions: To sum up, the present work introduced the purified laccase of P. variabile as an efficient biocatalyst
for removal of one of the most hazardous endocrine disruptor bisphenol A.
