Author/Authors :
Haji Ebrahim Zargar، Haleh نويسنده Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran , , Mohseni Meybodi، Anahita نويسنده Genetics Department, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , , Sabbaghian، Marjan نويسنده Department of Andrology, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , , Shahhoseini، Maryam نويسنده Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , , Asadpor، Ummulbanin نويسنده Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , , Sadighi Gilani، Mohammad Ali نويسنده , , Chehrazi، Mohammad نويسنده Department of biostatistics and epidemiology, Tehran university of medical sciences, Tehran, Iran , , Farhangniya، Mansoureh نويسنده Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , , Shahzadeh Fazeli، Seyed Abolhassan نويسنده Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran ,
Abstract :
Background: During spermatogenesis, the H2B family, member W (H2B.W) gene, encodes
a testis specific histone that is co-localized with telomeric sequences and has the
potential role to mediate the sperm-specific chromatin remodeling. Previously H2B.W
genetic variants were reported to be involved in susceptibility to spermatogenesis impairment.
In the present study, two single nucleotide polymorphisms (SNPs) in 5?UTR
and exon 1 of H2B.W gene were examined to investigate possible association of these
polymorphisms with male infertility in Iranian population.
Materials and Methods: This case control study was conducted in Royan institute during
four-year period (2010–2013). Genetic alteration of two SNPs loci, ?9C > T and 368A > G, in
H2B.W gene were indicated in 92 infertile men who were divided into two main groups including
azoospermia (n=46) and sever oligozoospermia (n=46), while there was 60 fertile men as
control group. Azoosperima was also divided into three sub-groups including sertoli cell only
syndrome (SCOS, n=21), complete maturation arrest (CMA, n=17) and hypo spermatogenesis
(n=8) according to testicular biopsy. For analysis, polymerase chain reaction-restriction fragment
length polymorphism (PCR-RFLP) technique was applied.
Results: The frequency of allele ?9T was significantly higher in CMA group than in
patients with SCOS (P < 0.05). The haplotype TA (corresponding to simultaneous occurrence
of ?9T and 368A) compared with haplotype CA (corresponding to simultaneous
occurrence of ?9C and 368A) in patients suffering from CMA significantly increased,
compared with patients had SCOS (P < 0.05). However, statistical studies indicated that in
general, the distribution frequencies of ?9C > T and 368A > G had no significant difference
between the infertile groups and control (P=0.859 and P=0.812, respectively).
Conclusion: This investigation showed that SNP ?9C > T might be contribute to CMA in azoospermic
patients and SNP 368A > G had no correlation with male infertility in Iranian population.
