Author/Authors :
Ravanipour، Masoumeh نويسنده Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , , Rezaei Kalantary، Roshanak نويسنده Department of Environmental Health, School of Health, Tehran University of Medical Sciences, Tehran, Iran. Rezaei Kalantary, Roshanak , Mohseni-Bandpi، Anoushiravan نويسنده Department of Environmental Health Engineering, School of Public Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Esrafili، Ali نويسنده Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , , Farzadkia، Mahdi نويسنده Associate Professor, Department of Environmental Health Engineering , , Hashemi-Najafabadi، Samireh نويسنده Department of Chemical Engineering, Biotechnology Group, Tarbiat Modares University, Tehran, Iran ,
Abstract :
Background: The effectiveness of bioremediation systems for PAH-contaminated soil may be constrained by
physicochemical properties of contaminants and environmental factors. Information on what is the most
effective factor in bioremediation process is essential in the decision of what stimulations can be taken to assist
the biodegradation efficacy.
Methods: In this study, four factors of surfactant (Tween 80), humic acid (HA), salinity and nutrients in a 24 full
factorial design were screened in bioremediation of phenanthrene contaminated soil by using a consortium of
bacteria.
Results: Between the employed levels of the factors only salinity had not significant effect. Optimal concentrations of
surfactant, HA and nutrient were obtained by a response surface design. For phenanthrene biodegradation, a central
composite face centred design (CCFD) showed that nutrient, surfactant and HA concentrations had highly significant,
significant and insignificant effects, respectively. The best conditions with 87.1% phenanthrene biodegradation were
150 mg HA/Kg soil, 12.68 ?g/L surfactant, and nutrients as K2HPO4, 0.8; KH2PO4, 0.2 and KNO3, 1 g/L. A high similarity
was between the model prediction and experimental results.
Conclusions: This study showed that nutrient with 81.27% efficiency could be considered as the most effective factor
for practical implications of bioremediation process for PAHs contaminated soil cleanup strategies.
