• Title of article

    Apparatus for X-ray diffraction microscopy and tomography of cryo specimens

  • Author/Authors

    Beetz، نويسنده , , T. and Howells، نويسنده , , M.R. and Jacobsen، نويسنده , , C. and Kao، نويسنده , , C.-C. and Kirz، نويسنده , , J. and Lima، نويسنده , , E. and Mentes، نويسنده , , T.O. and Miao، نويسنده , , H. and Sanchez-Hanke، نويسنده , , C. and Sayre، نويسنده , , D. and Shapiro، نويسنده , , D.، نويسنده ,

  • Issue Information
    روزنامه با شماره پیاپی سال 2005
  • Pages
    10
  • From page
    459
  • To page
    468
  • Abstract
    An apparatus for diffraction microscopy of biological and materials science specimens is described. In this system, a coherent soft X-ray beam is selected with a pinhole, and the illuminated specimen is followed by an adjustable beamstop and CCD camera to record diffraction data from non-crystalline specimens. In addition, a Fresnel zone plate can be inserted to allow for direct imaging. The system makes use of a cryogenic specimen holder with cryotransfer capabilities to allow frozen hydrated specimens to be loaded. The specimen can be tilted over a range of ± 80 ∘ for three-dimensional imaging; this is done by computer-controlled motors, enabling automated alignment of the specimen through a tilt series. The system is now in use for experiments in soft X-ray diffraction microscopy.
  • Keywords
    X-ray microscopy , x-ray imaging , X-ray tomography , Diffraction microscopy , lensless imaging
  • Journal title
    Nuclear Instruments and Methods in Physics Research Section A
  • Serial Year
    2005
  • Journal title
    Nuclear Instruments and Methods in Physics Research Section A
  • Record number

    2203647