Inverse Miniemulsion Method for Synthesis of Gelatin Nanoparticles in Presence of CDI/NHS as a Non-toxic Cross-linking System

در اين پژوهش نانوذرات ژلاتين به روش ميني امولسيون معكوس وبه كارگيري مخلوط كربودي ايميد (CDI) محلول در اب و N- هيدروكسي سوكسينيميد (NHS) به عنوان اتصال دهنده ي عرضي غير سمي سنتز شد. نانوذرات ژلاتين سنتز شده از لحاظ اندازه، توزيع اندازه، مورفولوژي و پايداري با ژلاتين سنتز شده با اتصال دهنده ي گلوتارالدهيد به عنوان اتصال دهنده ي مرسوم مقايسه گرديد. نتايج نشان داد كه هنگام استفاده از NHS نانوذرات همگن با اندازه ي كوچكتر حاصل مي شود و در نهايت مشخص شد كه ژلاتين حاصل از CDI/NHS گزينه ي مناسبي براي زمينه ي دارورساني مي باشد.

In this research, gelatin nanoparticles were synthesized via inverse miniemulsion method by employing a mixture of a water soluble carbodiimide (CDI) and N-hydroxysuccinimide (NHS) as a non-toxic cross-linking system. The gelatin nanoparticles were characterized for their size and size distribution, morphology and stability and were compared with those of nanoparticles cross-linked by glutaraldehyde (GA) as the most commonly utilized cross-linking agent. The results showed the formation of more homogeneous nanoparticles with smaller size when CDI/NHS used as cross-linking agent under the same synthesis condition. Moreover, dilute solution viscosimetry experiments confirmed the stability of the nanoparticles under various physicochemical conditions. The differences in the characteristics of CDI/NHS and GA cross-linked nanoparticles were ascribed to the different nature of network formation using the two cross-linking agents. Generally, these results suggested CDI/NHS cross-linked gelatin nanoprticles as an interesting candidate for drug delivery application.