Studies on blood biochemical constituents and rumen fermentation in growing buffalo calves fed ammoniated straw-based rations supplemented with different protein sources

Studies were done on blood biochemical constituents and rumen fermentation pattern of growing buffalo calves fed ammoniated wheat straw (UAS)-based rations supplemented with three different protein supplements. Eighteen buffalo calves (140 kg) were divided into three groups of six calves each and were fed on UAS supplemented with a concentrate mixture (roughage:concentrate ratio 58:42) containing 8% (or 3.5% of total ration) untreated groundnut cake (GNC) (group I), 8% formaldehyde treated GNC (group II) and 8% fish meal (group III), respectively, for 19 weeks. During this growth study, blood biochemistry was studied. Rumen fermentation study was done on three fistulated growing buffalo calves using a 3×3 Latin square switch over design. Blood glucose and ammonia-N did not differ significantly among the treatments. However, blood urea-N of calves in group III was higher (P<0.05) than in groups I and II, but the latter two did not differ from each other in this respect. Total protein in group I was comparable to group II and group III which were significantly different from each other. The rumen pH, total volatile fatty acids, ammonia-N and total protozoal count were similar, however, total N and TCA insoluble N in group II and group III were higher (P<0.01) than in group I. Fish meal containing diet favoured holotrichs, whereas, protected GNC containing diet favoured spirotrichs as indicated by significantly higher count. It was concluded that the beneficial effects of supplementing formaldehyde protected GNC at 8% in the concentrate mixture to the UAS-based diets were greater than supplementation of unprotected GNC or low quality fish meal in ration of buffalo calves growing at 400 g because of improved rumen fermentation, absorption and metabolism of nutrients as observed in blood biochemistry.