Author/Authors :
Ebrahimi Kasgari، Sedigheh نويسنده Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, IR Iran , , Nourani، Mahnaz نويسنده Infection Diseases Research Center, Babol University of Medical Sciences, Babol, IR Iran , , Yahyapour، Yousef نويسنده Infectious Disease and Tropical Center, Babol University of Medical Sciences, Babol, IR Iran , , Mousavi، Seyed Ehsanollah نويسنده Department of Microbiology, Babol University of Medical Sciences, Babol, IR Iran , , Kalantar ، Enayatollah نويسنده Envirronmental Health Resaerch Center,KurdistanniversityofMedical Sciences, Sanandaj, Iran Kalantar , Enayatollah , Kaboosi، Hami نويسنده Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, IR Iran , , Marashi، Seyed Mahmoud Amin Seyed Mahmoud Amin نويسنده Cellular and Molecular Biology Research Center, (CMBRC), Babol University of Medical Sciences, Babol, Iran Marashi, Seyed Mahmoud Amin Seyed Mahmoud Amin
Abstract :
Cholera is one of the most diseases of human. Cholera toxin is the most important pathogenic factor in humans that causes diarrhea. The cholera toxin is produced by V. cholerae and CTXфPhage. In this study, we have investigated the production cholera toxin with different density of Vibrio cholerae. With this propose we inoculated classical strain O1 of Vibrio cholerae ATCC 14035 and Vibrio cholerae O1biovar El Tor N16961 into the AKI medium. Then, the total mRNA was determined by standard procedure which was converted into total cDNA. Cholra toxin production was determined by qPCR and maximum production of cholera toxin was at 1010 cfu/mL. In conclusion, production of cholera toxin was minimized almost up to zero at 1010.5 cfu/mL; which could be due to presence of high level concentration of autoinducer.
