Phytochemical Analysis of Danae Racemosa L. Moench Leaves

Background: Danae racemosa (L.) Moench (Ruscus racemosa L., Asparagaceae) is an erect, muchbranched evergreen shrub, native in the mountains from Syria to Iran, commonly used for its decorative green foliage in fresh flower arrangements. Despite its importance, far too little attention has been paid to gather detailed documentations of chemical constituent present in extract of D. racemosa. Methods: In this study, leaves of D. racemosa were collected from north of Iran, next, extracted via maceration followed by partitioning through solvents of different polarity. The qualitative–quantitative chemical composition of the extract was determined using high-performance liquid chromatography. Results: Two major flavonoids, quercetin and kaempferol, were separated whilst their identities were confirmed by UV-visible, shift reagents and 1H-NMR spectroscopy.