Author/Authors :
Arjmand، Mohammad نويسنده Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran , , Mirzaei، Abdolah نويسنده Department of Clinical Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran , , Jafarzadeh Shirazi، Mohammad Reza نويسنده Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran , , Tamadon، Amin نويسنده Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University & Stem Cell and Transgenic Technology Research Center, Shiraz Uni Tamadon, Amin , Salehi، Mohammad Saied نويسنده Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran , , Saeb، Mehdi نويسنده Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran , , Namavar، Mohammad Reza نويسنده Histomorphometry and Stereology Research Center , Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz , , Zandi، Mohammad Reza Mohammad Reza نويسنده Department of Animal Sciences, School of Agriculture, Shiraz University, Shiraz, Iran. Zandi, Mohammad Reza Mohammad Reza , Shahheidari، Hojatollah نويسنده Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran , , Moradi، Sara نويسنده Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran ,
Abstract :
The aims of the present study were to evaluate luteal activity in Abadeh ecotype goat during summer and winter and also the effect of a single dose kisspeptin-10 injection on the secretion of luteinizing hormone (LH) in female anestrous goats. In the first study, progesterone (P4) concentration in 10 goats in summer (n = 6) and winter (n = 4) were measured every other day. Moreover, in summer group, a male teaser goat was left in the herd on days of sampling for one hour. Goats with P4 concentration ?1 ng mL-1, at least two consecutive measurements, were considered with luteal activity. In the second study, the anestrous phase was confirmed by P4 measurement 20 and 10 days before the kisspeptin injection in five female Abadeh ecotype goats (4 to 5 years old). The goats were given a single IV injection of saline (2 mL) as control group and the same goats (1 hr after the last blood sampling) were given kisspeptin (1 ?g kg-1) as treatment group. The blood samples were collected at –60, –40, –20 and 0 min (before injection), and 10, 20, 30, 40, 50, 60, 80, 100, 120 and 140 min after the injection and LH concentration were measured. A single IV injection of 1 µg kg-1 of kisspeptin-10 did not stimulate the release of LH in female anestrous goats. In summer, in the presence of teaser goat, luteal activity was seen in all goats. In the absence of male goat in winter, some goats showed luteal activity and others showed anestrus.
