Author/Authors :
Della Torre، نويسنده , , Camilla and Tornambè، نويسنده , , Andrea and Cappello، نويسنده , , Simone and Mariottini، نويسنده , , Michela and Perra، نويسنده , , Guido and Giuliani، نويسنده , , Silvia and Amato، نويسنده , , Ezio and Falugi، نويسنده , , Carla and Crisari، نويسنده , , Antonella and Yakimov، نويسنده , , Michail M. and Magaletti، نويسنده , , Erika، نويسنده ,
Abstract :
The aim of this study was to assess medium-term toxicity of weathered oil on European seabass. A mesocosm system reproducing an oil spill at sea was applied. Fish were collected after 48 h, 7, 30 and 60 days. Cyp1a gene transcription, EROD and UDPGT activities, bile PAHs metabolites and micronuclei frequency were investigated. A progressive disappearance of low molecular weight n-alkanes and PAHs in the water of the mesocosm occurred during the experimentation. Fishes exposed to oil displayed a significant increase of cyp1a expression and EROD activity during the entire experiment as well as higher concentrations of PAHs metabolites in bile. Micronulei frequency resulted significantly higher during all experiment in oil exposed sea bass compared to controls. The results highlight the environmental risk associated with the release of oil products at sea and confirm the adopted parameters as useful tools for studying the impact of accidental oil spills on fish.
Keywords :
mesocosm , Micronuclei , European seabass , Weathered oil , EROD , Bile PAH metabolites
