Title of article
Extracting DNA from within intact foraminiferal shells
Author/Authors
Seears، نويسنده , , Heidi A. and Wade، نويسنده , , Christopher M.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2014
Pages
8
From page
46
To page
53
Abstract
Here we present an evaluation of several methods for extracting DNA from within intact foraminiferal shells. We tested 12 lysis buffers under carefully optimised incubation conditions, evaluating their success in terms of efficiency in evacuating cellular material, crude DNA yield, PCR success, and final shell integrity. A number of the buffers tested produced excellent results. The most successful method utilized a lysis buffer containing Sodium N-lauroyl sarcosine, Guanidinium isothiocyanate, Isopropanol, TRIS and NaCl, incubated at 75 °C for 24 h, followed by chloroform extraction and ethanol precipitation. High yields of DNA were produced, with no signs of PCR inhibition, and the foraminiferal shells were left completely intact. Retaining the shell of individual specimens presents a significant advance, allowing for direct comparisons between shell morphology and genotype data, which could greatly enhance the utility of foraminifera as palaeoproxies of past climate change.
Keywords
Foraminifera , DNA extraction , Shell preservation , Cell lysis , PCR Inhibition
Journal title
Marine Micropaleontology
Serial Year
2014
Journal title
Marine Micropaleontology
Record number
2264308
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