Author/Authors :
Saneipour، Maryam نويسنده MSc Student, Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran , , Ghatreh-Samani، Keihan نويسنده Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. Ghatreh-Samani, Keihan , Heydarian، Esfandiar نويسنده Associate Professor, Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran , , Farrokhi، Effat نويسنده Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. Farrokhi, Effat , Abdian، Narges نويسنده PhD Candidate, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran ,
Abstract :
BACKGROUND: High expression of matrix metalloproteinase 9 (MMP9) during vascular injury
and inflammation plays an important role in atherosclerotic plaque formation and rupture. In
the process of atherosclerosis, oxidized low-density lipoprotein (oxLDL) upregulates MMP9 in
human aortic vascular smooth muscle cells (HA/VSMCs). Adiponectin is an adipose tissuederived
hormone that has been shown to exert anti-atherogenic and anti-inflammatory effects.
The aim of this study was to investigate the effect of adiponectin on MMP9 expression under
pathogenic condition created by oxLDL in HA/VSMCs.
METHODS: In this experimental study, HA/VSMC were stimulated with oxLDL alone and in the
presence of adiponectin for 24 and 48 h. The expression of MMP9 gene was determined by realtime
polymerase chain reaction method. The protein level of this gene was investigated by
western blotting technique.
RESULTS: An oxLDL increased MMP9 expression 2.16 ± 0.24- and 3.32 ± 0.25-fold after 24 and
48 h, respectively and adiponectin decreased oxLDL-induced MMP9 expression in a timedependent
manner.
CONCLUSION: These results show that adiponectin changes extracellular matrix by reducing
MMP9 mRNA and protein, therefore, may stabilize lesions and reduce atheroma rupture.
