Title of article :
Effect of N-acetyl cysteine on orthodontic primers cytotoxicity
Author/Authors :
D’Ant?، نويسنده , , Vincenzo and Spagnuolo، نويسنده , , Gianrico and Schweikl، نويسنده , , Helmut and Rengo، نويسنده , , Sandro and Ambrosio، نويسنده , , Luigi and Martina، نويسنده , , Roberto and Valletta، نويسنده , , Rosa، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Abstract :
Objectives
ms of this study were to evaluate the cytotoxicity of four orthodontic primers, including two hydrophilic and two hydrophobic materials, and to investigate the role of the reactive oxygen species (ROS) in induced cell damage. Moreover, the effects of the anti-oxidant N-acetyl cysteine (NAC) on primers toxicity was analyzed.
s
gingival fibroblasts (HGF) were exposed to different concentrations of primers (0–0.25 mg/ml) in the presence or absence of NAC, and the cytotoxicity was assessed by the MTT assay, while cell death was quantified by flow cytometry after propidium iodide staining. The increase in the induced ROS levels was detected by flow cytometry measuring the fluorescence of the oxidation-sensitive dye 2′,7′-dichlorofluorescein diacetate (DCFH-DA).
s
terials decreased cell viability in a dose-related manner after a 24 h exposure period. Cytotoxicity of orthodontic primers based on concentrations which caused a 50% decrease in cell viability (TC50) in HGF was ranked as follows (median values): Eagle Fluorsure (0.078 mg/ml) > Transbond XT (0.081 mg/ml) > Transbond MIP (0.128 mg/ml) > Ortho solo (0.130 mg/ml). Moreover, in HGF cells, all materials induced a dose-dependent increase in ROS levels compared to untreated cells. Incubation of HGF with NAC significantly reduced ROS production and decreased the cell damage and cytotoxicity caused by all materials tested (p < 0.001).
icance
sults suggested that hydrophilic primers were less cytotoxic than hydrophobic materials. Moreover, we demonstrated a major role of ROS in the induction of cell death since the antioxidant N-acetyl cysteine was able to prevent cell damage induced by all materials tested.
Keywords :
Reactive oxygen species , cytotoxicity , N-Acetyl Cysteine , Orthodontic adhesives
Journal title :
Dental Materials
Journal title :
Dental Materials