Author/Authors :
Rehman، Sadia نويسنده Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan , , Saba، Nusrat نويسنده Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan , , Akhtar، Naveed نويسنده , , Munir، Saeeda نويسنده Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan , , Waqar، Ahmed نويسنده Armed Forces Institute of Cardiology and National Institute of Heart diseases , , Khanum، Azra نويسنده Pir Mehr Ali Shah Arid Agriculture University, Rawlpindi, Pakistan ,
Abstract :
Objectives: The present research work aimed to assess the role of ACE Insertion/Deletion (I/D) polymorphism in progress of RHD.
Patients and Methods: DNA was pre pared from blood samples from 156 RHD patients (156) and 204 healthy ethnically-matched controls. Then, it was screened using sequence-specific Primers. Polymerase chain reaction and Agarose gel electrophoresis. The data were analyzed using Vassar stats (http://faculty.vassar.edu/lowry/VassarStats.html).
Results: I allele (P = 0.024, OR = 1.42) and II genotype (P = 0.001, OR = 3.07) were significantly higher in Pakistani RHD patients compared to the healthy controls. Also, a significant difference was found between the female, but not male, patients and the controls regarding I allele and II genotype.
Conclusions: The study results provided information about involvement of ACE I/D polymorphism in molecular mechanism of RHD. Thus, it can become one of the useful tools in risk assessment and help with designing strategies to combat the disease.
