Author/Authors :
Mortazavi، S.M.J. نويسنده , , Erfani، N. نويسنده Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, I.R Iran. , , Mozdarani، H. نويسنده Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran , , Azmoonfar، R. نويسنده Radiology Department, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran Azmoonfar, R. , Shokrpour، N. نويسنده School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran Shokrpour, N.
Abstract :
Background: Radiation-induced bystander effect is a response which results in damage in non-irradiated cells in response to signals from the irradiated cells. The aim of the present study was to investigate microwave-induced bystander effect from a GSM mobile phone simulator on induction of apoptosis in Jurkat cell line. Materials and Methods: Jurkat cells were divided into three groups of non-irradiated, exposed and bystander (medium transfer from the irradiated cells). The exposed group was subjected to irradiation from GSM mobile simulator for 2h and 12h; the medium from irradiated cells was then transferred to the bystander cells. Apoptosis rate was measured12 and 24 hours after treatment by Annex in V 7-AAD kit using flow cytometry. Results: Apoptosis was observed in both exposed and bystander cells of Jurkat cell line. The difference among non-irradiated, exposed and bystander cell groups was significant (p<0.05). Conclusion: From the obtained results it can be concluded that microwave radiation exposure in Jurkat cells leads to a significant increase in the apoptosis rate not only in the exposed cells but also in the bystander cells.
