Author/Authors :
-، - نويسنده Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara-Iran. Abdolahnejad, Zeinab , -، - نويسنده Iran Fishery Research Organization (IFRO), Tehran, Iran. Pourkazemi, Mohammad , -، - نويسنده Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara-Iran. Khoshkholgh, Majid Reza , -، - نويسنده International Sturgeon Research Institute, Rasht-Iran. Yarmohammadi, Mahtab
Abstract :
The mRNA expression of growth hormone (GH) gene in early development stages of Siberian sturgeon was investigated using RT-PCR method. Samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. Ribosomal protein L6 (RPL6) transcripts were used as the internal standard during quantification of GH mRNA expression. The results showed that the GH mRNA could be observed in the eyed eggs and even at unfertilized eggs of Siberian sturgeon. The highest amounts of GH mRNA were found at 25 and 50 dph larvae, while the lowest levels were detected at 1 and 3 dph larvae stage. These findings suggest that, the GH mRNA play a key role during developmental stages of Siberian sturgeon.
