Author/Authors :
Karimfar ، Mohammad Hassan نويسنده Department of Anatomy, School of Medicine, Ilam University of Medical Sciences, Ilam- Iran. , , Peyvandi، Aliasghar نويسنده Hearing Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Peyvandi, Aliasghar , Noorozian، Mohsen نويسنده Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Noorozian, Mohsen , Ahmadi Roozbahani، Navid نويسنده Hearing Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Ahmadi Roozbahani, Navid , Mastery Farahani، Reza نويسنده Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Mastery Farahani, Reza , Khoramgah، Maryam Sadat نويسنده Department of Medical Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Khoramgah, Maryam Sadat , Azimi، Hadi نويسنده PhD student , , Bahadori Monfared، Ayad نويسنده Department of Epidemiology, Faculty of Health, Shahid Beheshti University of Medical Sciences, P.O. Box 19395-4741, Tehran, I.R. Iran , , Abbaszadeh، Hojjat-Allah نويسنده Department of Health & Community Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Abbaszadeh, Hojjat-Allah
Abstract :
Introduction: SOX9 is a transcriptional activator which is necessary for chondrogenesis. SOX6 are closely related to DNA-binding proteins that critically enhance its function. Therefore, to carry out the growth plate chondrocyte differentiation program, SOX9 and SOX6 collaborate genomewide. Chondrocyte differentiation is also known to be promoted by glucocorticoids through unknown molecular mechanisms.
Methods: We investigated the effects of asynthetic glucocorticoid, dexamethasone (DEX), on SOX9 gene expression in chondrocytes.
Results: SOX9 mRNA was expressed at high levels in these chondrocytes. Treatment with DEX resulted in enhancement of SOX9 mRNA expression. The DEX effect was dose dependent (0·5 nM and 1 nM).
Conclusion: RT-PCR analysis revealed that DEX also enhanced the levels of SOX9 expression. It was observed that DEX had enhancing effect only on SOX9; the expression level was low for SOX6. It can thus be concluded that chondrocyte differentiation can be promoted by DEX via SOX9 enhancement.
