Title of article :
Interleukin-1 beta, interferon-gamma, and tumor necrosis factor-alpha gene expression in peripheral blood mononuclear cells of patients with coronary artery disease
Author/Authors :
Enayati، Samaneh نويسنده Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran , , Seifirad، Soroush نويسنده Research Fellow, Department of Cardiology, Children Medical Center, Tehran University of Medical Sciences, Tehran, Iran , , Amiri، Parvin نويسنده Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran , , Abolhalaj، Milad نويسنده Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran , , Mohammad -Amoli، Mahsa نويسنده Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran ,
Abstract :
BACKGROUND: Several inflammatory mediators have been proposed to contribute to the
pathogenesis of atherosclerosis. The aim of this study was to evaluate the quantitative
expression of pro-inflammatory cytokines in un-stimulated peripheral blood mononuclear cell
of patients with coronary artery disease (CAD).
METHODS: Interleukin-1 beta (IL-1B), tumor necrosis factor-alpha, and interferon-gamma
(IFN-?) gene expression were evaluated in angiography confirmed patients with and without
CAD in a case-control study using quantitative real-time polymerase chain reaction.
RESULTS: A significant increase (P = 0.030) in IL-1B gene expression was found in patients with
CAD [median interquartile range (IQR) = 4.890 (6.084)] compared to patients without CAD
[median (IQR) = 1.792 (3.172)]. Despite the increase in IFN-? gene expression in patients with
CAD [median (IQR) = 1.298 (3.896)] versus patients without CAD [median (IQR) = 0.841
(2.79)], there was not statistically significant difference (P = 0.990).
CONCLUSION: Our results provide evidence for possible association between IL-1B and
development of atherosclerosis as a crucial cytokine that induce a network of signaling pathways.
This finding if proved in future would suggest IL-1B as a potent therapeutic target in CAD.
