Author/Authors :
Askari، Mohammad نويسنده Department of Medical Biotechnology, Faculty of Allied Medical Sciences, Tehran University of Medical Sciences, P.O. Box 1449614535, Tehran, I.R. Iran , , Nikpoor، Amin Reza نويسنده Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran. , , Aryan، Hajar نويسنده Fazeli-Sanati Genetic Laboratory, Tehran, Iran , , Ghaedi، Hamid نويسنده Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Akhtari، Javad نويسنده Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Akhtari, Javad , Azarnejad، Asaad نويسنده Department of Medical Biotechnology, Tehran University of Medical Sciences, Tehran, Iran Azarnejad, Asaad , Mousavizadeh، Kazem نويسنده Cellular and Molecular Research Center, Tehran University of Medical Sciences (TUMS), Tehran, Iran. , , Sanati، Mohammad Hossein نويسنده , , Irani، Alireza نويسنده Eye Research Center, Rassoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran , , Ghasemi-Falavarjani,، Khalil نويسنده , , Nazari، Hossein نويسنده ,
Abstract :
Background: We aimed here to investigate the association between alleles and genotypes of APOE and Age-related macular degeneration (AMD) development.
Materials and Methods: After ophthalmological examination, 120 patients with confirmed AMD and 120 healthy controls were enrolled in the study. The polymorphic segment of APOE gene was PCR-amplified and sequenced to determine the frequency distribution of polymorphic alleles and genotypes of this gene in the sample population.
Results: The frequency distribution of APOE alleles and genotypes differed significantly between the patients and the control groups (P<0.05). The frequency of APOEε2 in patients was higher than controls (P = 0.00) and this variant allele showed a significant association with AMD even after removal of the effects of age, sex and smoking by logestic regression analysis (P = 0.00, OR= 3.439; CI 95% 1.664-7.108). On the other hand, the frequency distribution of APOEε4 was not statistically different between patients and healthy controls.
Conclusion: The results show a moderate positive association between APOE ε2 and AMD, but current data does not show any role for APOE ε4 in protection from AMD. However, more studies are required to reveal the possible role of APOE in the pathogenesis of AMD.
