Author/Authors :
Nasrollahi، Zahra نويسنده Department of Economic, College of Economic, Management and Accounting Yazd University, Iran , , Roudbar Mohammadi، Shahla Roudbar نويسنده Department of Mycology, Tarbiat Modares University of Medical Science, Tehran , , Nikoomanesh، Fatemeh نويسنده Religion and Medicine Research center, Qom University of Medical Sciences, Qom, IR Iran , , Yadegari، Mohammad Hossein نويسنده Department of Medical Mycology, Faculty of Medicine, Tarbiat Modares University, Tehran, IR Iran , , Roudbary، Maryam نويسنده Department of Medical Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Roudbary, Maryam , Rajabi Bazl، Masumeh نويسنده Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran ,
Abstract :
Background: Some genes may be associated with Candida albicans resistance to azoles. Pir1 gene is described as responsible to induce resistance in C. albicans.
Objectives: The current study aimed to find the relationship between fluconazole resistance and Pir1 protein (Pir1p) overexpression in the females with recurrent C. albicans vaginitis requiring longer fluconazole therapy.
Patients and Methods: A total of 52l vaginal samples were obtained from the females with C. albicans vaginitis. The azole susceptibility phenotype was determined according to the Clinical Laboratory for Standards Institute (CLSI) protocol for disk diffusion method and inhibition zone for fluconazole. Expression of pir1 gene and fluconazole -resistance were evaluated using polymerase chain reaction (PCR) in C. albicans.
Results: In the 52 isolates, 49 (94%) were resistant to fluconazole. Overexpression of Pir1 gene was detected in 47 (96%) fluconazole-resistant C. albicans isolates.
Conclusions: The findings show fluconazole -resistance in C. albicans isolates with overexpression of Pir1p.
