Title of article
Morphological and Genotypic Variations among the Species of the Subgenus Adlerius (Diptera: Psychodidae, Phlebotomus) in Iran
Author/Authors
Zahraei-Ramazani، Alireza نويسنده Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Zahraei-Ramazani, Alireza , Kumar، Dinesh نويسنده , , Mirhendi، Hossein نويسنده Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mirhendi, Hossein , Sundar، S. Shyam نويسنده , , Mishra، Rajnikan نويسنده Department of Zoology, Centre of Advanced Study, Banaras Hindu University, Varanasi 221 005, India. Mishra, Rajnikan , Moin-Vaziri ، Vahideh نويسنده Department of Parasitology and Mycology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Soleimani، Hassan نويسنده , , Shirzadi، Mohammadreza نويسنده Department of Zoonosis, CDC of Iran, Ministry of Health, Tehran, Iran , , Jafari، Reza نويسنده Department of Ophthalmology, Zahedan University of Medical Sciences, Zahedan, Iran Jafari, Reza , Hanafi-Bojd، AhmadAli نويسنده Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Hanafi-Bojd, AhmadAli , Hamedi-Shahraky، Sodabe نويسنده School of Health, Zabol University of Medical Sciences, Zabol, Iran. Hamedi-Shahraky, Sodabe , Yaghoobi-Ershadi، MohammadReza نويسنده Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Yaghoobi-Ershadi, MohammadReza
Issue Information
فصلنامه با شماره پیاپی 0 سال 2015
Pages
14
From page
84
To page
97
Abstract
Background: Female sand flies of subgenus Adlerius are considered as probable vectors of visceral leishmaniasis in Iran. The objective of this study was to determine the morphological and genotypic variations in the populations of this subgenus in the country.
Methods: Sand flies collected using sticky traps from 17 provinces during 2008–2010. The morphometric measurements were conducted with an Ocular Micrometer. Data was analyzed by SPSS. The Cytb gene was used to estimate population genetic diversity and identify the female specimens. UPGMA phenetic tree was used for DNA haplotypes of Cytb gene.
Results: Six species of subgenus Adlerius identified from which one species, P. (Adlerius) kabulensis, is new record. The identification key is provided for males. Results revealed the molecular systematic in the species of subgenus Adlerius and determine the relationship of three females of P. comatus, P. balcanicus and P. halepensis.
Conclusion: The positions of three females and the males in the UPGMA tree are correct and the similarities among them confirm our results. The branches of each species are not genetically distinct which justify the overlapping morphological characters among them. Molecular sequencing of Cytb-mtDNA haplotypes can be used for female identification for different species of subgenus Adlerius in Iran.
Methods: Sand flies collected using sticky traps from 17 provinces during 2008–2010. The morphometric measurements were conducted with an Ocular Micrometer. Data was analyzed by SPSS. The Cytb gene was used to estimate population genetic diversity and identify the female specimens. UPGMA phenetic tree was used for DNA haplotypes of Cytb gene.
Results: Six species of subgenus Adlerius identified from which one species, P. (Adlerius) kabulensis, is new record. The identification key is provided for males. Results revealed the molecular systematic in the species of subgenus Adlerius and determine the relationship of three females of P. comatus, P. balcanicus and P. halepensis.
Conclusion: The positions of three females and the males in the UPGMA tree are correct and the similarities among them confirm our results. The branches of each species are not genetically distinct which justify the overlapping morphological characters among them. Molecular sequencing of Cytb-mtDNA haplotypes can be used for female identification for different species of subgenus Adlerius in Iran.
Journal title
Journal of Arthropod-Borne Diseases
Serial Year
2015
Journal title
Journal of Arthropod-Borne Diseases
Record number
2353081
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