Author/Authors :
RASOLZADEH، Sanaz نويسنده Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran RASOLZADEH, Sanaz , HAJI FATAHALIHA، Mostafa نويسنده Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran HAJI FATAHALIHA, Mostafa , HOSSEINI، Maryam نويسنده Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran HOSSEINI, Maryam , JAFARI، Reza نويسنده Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran JAFARI, Reza , MIAHIPOUR، Abolfazl نويسنده Dept. of Medical Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran MIAHIPOUR, Abolfazl , SADREDDINI، Sevil نويسنده Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran SADREDDINI, Sevil , BABALO، Zohreh نويسنده Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran BABALO, Zohreh , SAMADI KAFIL، Hossein نويسنده Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran SAMADI KAFIL, Hossein , yousefi ، Mehdi نويسنده ,
Abstract :
Background:Natural killer (NK) cells play an important role in early stages of innate immune responses against viral and tumoral attacks. Activation of NK cells by leishmaniasis results in secretion of cytokines such as interferon (IFN)-γ and tumor necrosis factor (TNF)-α, which enhance the phagocytosis and clearance of parasite. Lipophosphoglycan 3 (LPG3), the Leishmania homologous with GRP94 (glucose regulated protein 94), a member of HSP90 family, contributes to LPG assembly as the most abundant macromolecule on the surface of Leishmania promastigotes.
Methods:We purified NK cells from healthy individuals (n=10) using magnetic-activated cell sorting (MACS) technology. Purified NK cells were co-incubated with different concentrations of recombinant LPG3 (rLPG3), and its N-terminal (NT) and C-terminal (CT) fragments. Finally, the production of IFN-γ and TNF-α by NK cells were measured by ELISA.
Results:Recombinant LPG3 but not its fragments (CT and NT), could significantly enhance the production of TNF-α by NK cells (P<0.05). Moreover, rLPG3, CT, and NT fragments were markedly stimulated the secretion of IFN-γ by NK cells (P<0.001).
Conclusion: The
Leishmania LPG3 antigen could effectively activate NK cells, in vitro.
Leishmania LPG3 participates in the innate immunity against leishmaniasis and thereby improves the effective parasite destruction.However, its efficiency should be tested in vivo.
