Identification and Characterization of a Differentially Expressed Gene (07E12) in the Infective Larvae of the Parasitic Nematode Ascaris suum.
HUANG، Cuiqin نويسنده College of Life Science, Longyan University, Longyan, 364000, China; AND Key Laboratory of Preventive Veterinary Medicine and Biotechnology of Fujian Province, Longyan, 364000, China HUANG, Cuiqin , You، Jingzhou نويسنده College of Life Sciences, Longyan University, Longyan, China AND Longyan Entry-Exit Inspection and Quarantine Burean of the Peoples Republic of China, Longyan, China. You, Jingzhou , Nai، Fangfang نويسنده College of Life Sciences, Longyan University, Longyan, China. Nai, Fangfang
Background: Parasitic nematodes cause animal and human diseases of major socio-economic importance worldwide. The suppression of parasite development at particular developmental stages could provide an alternative approach for nema-tode control. In this study, Ascaris suum was used as a model system in the study of the differentially expressed genes in the infective L3 stage.
Methods: The gene (07E12) was screened and identified from the subtractive cDNA library for the infective larvae of Ascaris suum using real-time quantitative PCR. Then, the full-length cDNA of 07E12 was characterized by 3′ and 5′ rapid amplification of cDNA ends (RACE). The characteristics of the gene were further analyzed using bioinformatic analyses.
Results: The results showed that the gene 07E12 was differentially expressed in the third-stage larvae of A. suum and its expression level in the infective larvae was much higher than in other stages. It was shown that the gene 07E12 had 99% iden-tity with the corresponding sequences of the A. suum whole genome shotgun se-quence containing the homologous sequences with conserved sequences of Neu-ropeptide-Like Protein family member. Likewise, by performing BLASTN and BLASTP searches in the GenBank™, it was shown that this gene had 99 % identity with A. suum cre-nlp-2 protein.
Conclusion: This gene 07E12 which is differentially expressed in the third-stage larvae of A. suum may encode a neuropeptide-like protein family member, a very important molecule in the process of infecting a host.