Ficolin-A Enhances Inhibition of the C-Terminal 19 kDa Region of Merozoite Surface Protein-1 of Plasmodium berghei Using Test In Vivo.
Chen، F نويسنده The Faculty of Life Sciences, Hubei University, 368 Youyi Road, Wuchang, Wuhan 430062, China Chen, F , Liu، Q نويسنده Hainan Provincial Key Laboratory of Tropical Medicine, Pharmacy School, Hainan Medical College, Haikou 571199, China Liu, Q , Xue، Y نويسنده Lab of Medical Engineering, College of Medical Technology and Engineering, Henan University of Science and Techology, Luoyang 471003, China Xue, Y , Huang، Yh نويسنده Hainan Provincial Key Laboratory of Tropical Medicine, Pharmacy School, Hainan Medical College, Haikou 571199, China Huang, Yh , Huang، Fy نويسنده Hainan Provincial Key Laboratory of Tropical Medicine, Pharmacy School, Hainan Medical College, Haikou 571199, China Huang, Fy , Lin، Y نويسنده Hainan Provincial Key Laboratory of Tropical Medicine, Pharmacy School, Hainan Medical College, Haikou 571199, China Lin, Y , Tan، Gh نويسنده Hainan Provincial Key Laboratory of Tropical Medicine, Pharmacy School, Hainan Medical College, Haikou 571199, China Tan, Gh , Zhou، J نويسنده Wuhan Tuberculosis Dispensary, 28 Baofeng Road, Qiaokou, Wuhan, 430030, China Fan Chen and Qiang Liu are co-primary authors Zhou, J
Background: Malaria remains a serious public health problem with significant morbidity and mortal-ity. This study was conducted to identify whether ficolin-A could play an active role of against mala-ria infection.
Methods: The function of ficolin-A was analyzed in mouse model. The open reading frame of fico-lin-A was cloned from the liver of new born C57BL/6 mice by RT-PCR and then inserted into the expression vector of eukaryon to construct pVAX1-ficolin-A plasmid. Meanwhile, the open reading frame of the 19-kDa fragment of merozoite surface protein-1 of Plasmodium berghei (MSP119) was cloned and then the expression vector of eukaryon, pVAX1- MSP119 was constructed. Both recom-binant vectors were used in the mouse model of infection by Plasmodium berghei.
Results: pVAX1-ficolin-A alone could not significantly suppress parasite density and prolong sur-vival time of infection mice; however, when injected pVAX1-ficolin-A and pVAX1- MSP119 together, the percent of invasion by Plasmodium was decreased (from 43.78% to 22.23% at 10 day after infec-tion, compared to vector ) and the survival time was prolonged significantly in the infection mouse model (P=0.01).
Conclusion: Ficolin-A can enhance the immunoprotection of MSP119, it implies ficolin-A may be used as immunoenhancer in the study of vaccine defending malaria.