Double-Stranded RNA Viral Infection in Tehran Trichomonas vaginalis Isolates.
Heidary، S نويسنده Dept. of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Heidary, S , Bandehpour، M نويسنده Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Dept. of Biotechnology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Bandehpour, M , Valadkhani، Z نويسنده Dept. of Parasitology, Pasteur Institute of Iran, Tehran, Iran Valadkhani, Z , Seyyed-Tabaee، Sj نويسنده Dept. of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Seyyed-Tabaee, Sj , Haghighi، A نويسنده Dept. of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Haghighi, A , Abadi، AR نويسنده , , Kazemi، B نويسنده Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Dept. of Biotechnology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Kazemi, B
Background: Trichomonas vaginalis is a pathogenic protozoon and may be contaminated with dsRNA virus called Trichomonas vaginalis virus (TVV). The viral infection is an important factor for its patho-genesis and sensitivity to metronidazole. The presence of TVV is associated with qualitative and quantitative expression of cysteine proteinases and surface immunogenic; P270. The purpose of this study was to determine TVV frequency in T. vaginalis clinical isolates in Tehran, Iran.
Methods:The 46 T. vaginalis isolates were collected from Tehran Province and cultured in TYI-S-33 culture medium. Viral RNA was extracted and RT-PCR was done.
Results:Of 46 T. vaginalis isolates, 8 isolates (17.39%) were infected with TVV-1. There was not any association between patient age and TVV- infected T. vaginalis. There were 17.39% viral infection in T. vaginalis isolates which was lower than that reported by other researchers.
Conclusion:This is the first report on T. vaginalis isolates infection by TVV-1 in Iran.