Author/Authors :
Serpil، Can نويسنده Department of Physiology, Medical Faculty, Kafkas University, Kars, Turkey Serpil, Can , Gulsen، Cigsar نويسنده Department of Emergency Medicine, Medical Faculty, Kafkas University, Kars, Turkey Gulsen, Cigsar , Ozabacigil Fatma، Gur نويسنده Department of Medical Laboratory Techniques, Vocational School of Health Services, Ataturk University, Erzurum, Turkey Ozabacigil Fatma, Gur , Karamese Selina، Aksak نويسنده Department of Histology and Embryology, Medical Faculty, Kafkas University, Kars, Turkey Karamese Selina, Aksak , Jale، Selli نويسنده Department of Histology and Embryology, Medical Faculty, Ataturk University, Erzurum, Turkey Jale, Selli , Gulsum، Bacak نويسنده Department of Physiology, Medical Faculty, Ataturk University, Erzurum, Turkey Gulsum, Bacak , Semin، Gedikli نويسنده Department of Histology and Embryology, Veterinary Faculty, Ataturk University, Erzurum, Turkey Semin, Gedikli , Sahin، Gonul Zisan نويسنده Department of Medical Biology, Medical Faculty, Kafkas University, Kars, Turkey Sahin, Gonul Zisan , Serdar، Yigit نويسنده Department of Emergency Medicine, Medical Faculty, Kafkas University, Kars, Turkey Serdar, Yigit , Ismail، Can نويسنده Department of Histology and Embryology, Medical Faculty, Kafkas University, Kars, Turkey Ismail, Can , Mustafa، Gul نويسنده Department of Physiology, Medical Faculty, Ataturk University, Erzurum, Turkey Mustafa, Gul
Abstract :
Liver is one of the most important organs affected by exercise. According to the literature a few study to date has investigated the effects of estrogen supplementation on exercise-induced oxidative stress in liver tissue of rats. We aimed to investigate the effects of estrogen supplementation on oxidative stress markers in liver tissue of exercised rats. Male rats (n = 35) were divided as estrogen supplemented (n = 18) and non-supplemented groups (n = 17); these groups were further divided as rest and eccentric exercised groups. Eccentric exercise groups were further divided as rats killed after 1 hour and 48 hours of eccentric exercise. Estrogen (10 mg/kg) was administered subcutaneously for 30 days. Eccentric exercise was applied as treadmill run (15° downhill, 20 m/min) consisting of periods of "5 min" run and 2 min rest repeated 18 times. The rat liver was examined biochemically and histologically. Activities of GST, GSH-Px, CAT, SOD and MDA concentration were also measured spectrophotometrically. Some disruptions were detected in experimental groups compared with the control group. Additionally, exercise training caused an increase in SOD and decrease in GSH-Px activities in some experimental groups. SOD activities increased significantly in group 3 (Estrogen (-), eccentric exercise (+) killed (after 1 h), compared with group 5 (Estrogen (-), eccentric exercise (+) killed (after 48 h). On the other hand, GSH-Px activities were also significantly decreased in groups 3, 4 and 5 compared with the control group. Leukocyte infiltration in liver increased after 48 hours compared with after 1 hour and estrogen supplementation was not able to prevent this infiltration. Estrogen seemed to be not very effective to prevent eccentric exercise-induced liver damage.
