MtDNA CytB Structure of Rhombomys opimus (Rodentia: Gerbellidae), the Main Reservoir of Cutaneous Leishmaniasis in the Borderline of Iran-Turkmenistan.
Bakhshi، Hasan نويسنده Department of Medical Entomology and Vector Control, School of Public Health and National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran. Bakhshi, Hasan , Oshaghi، Mohammad Ali نويسنده Tehran University of Medical Sciences, Tehran, Iran. , , Abai، Mohammad Reza نويسنده , , Rassi، Yavar Rassi نويسنده Tehran University of Medical Sciences, Tehran, Iran. , , Akhavan، Amir Ahmad نويسنده Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Akhavan, Amir Ahmad , Mohebali، Mehdi نويسنده Department of Medical Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran , , Hajaran، Homa نويسنده Department of Medical Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Hajaran, Homa , Mohtarami، Fatemeh نويسنده Department of Medical Entomology and Vectors Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Mohtarami, Fatemeh , Mirzajani، Hossein نويسنده Sarakhs Health Center, Sarakhs, Razavi Khorasan Province, Iran. Mirzajani, Hossein , Maleki Ravasan ، Naseh نويسنده Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran ,
Background: Great gerbils, Rhombomys opimus, are the main reservoir host of zoonootic cutaneous leishmaniasis (ZCL) in Iran and neighboring countries. Based on morphological traits two subspecies R. opimus sodalis and R. opimus sargadensis have reported in the country. However, variation in infection rate and signs to Leishmania parasites, phenotype, size, and sexual polymorphisms demand more details to elucidate clearly the role of great gerbils in ZCL epidemiology.
Methods: PCR-RFLP and PCR-direct sequencing were used to analyze mitochondrial DNA cytochrome B (mtDNA- cytB) gene structure of R. opimus collected from Golestan and Khorasan-e-Razavi Provinces in 2011 that are neighbor to Turkmenistan Country where ZCL is endemic in both sides of the borderline.
Results: All of the specimens (n= 61) were morphologically or genetically similar to the typical R. opimus sodalis. However, there were 9 (1.5%) DNA substitutions throughout the 583 bp of the Cyt b gene of the samples sequenced comprising six DNA haplotypes. Maximum likelihood or neighbor joining phylogenetic trees inferred from the sequences could resolve the populations according to their subspecies as well as geographical origins.
Discussion: The DNA polymorphisms in the great gerbils may correspond to the signs and infection rate in the animal. However, further studies are needed to match these six haplotypes with different signs and parasite sustaining following infection with L. major in the great gerbils.