Author/Authors :
Basir، R. نويسنده Department of Human Anatomy, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400
Serdang, Selangor, Malaysia. Basir, R. , Fazalul-Rahiman، SS. نويسنده Department of Human Anatomy, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400
Serdang, Selangor, Malaysia. Fazalul-Rahiman, SS. , Hasballah، K. نويسنده Department of Pharmacology, Faculty of Medicine, Syiah Kuala University, 23111 Darussalam, Banda Aceh,
Nanggroe Aceh Darussalam, Indonesia. Hasballah, K. , Chong، WC. نويسنده Department of Human Anatomy, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400
Serdang, Selangor, Malaysia. Chong, WC. , Talib، H. نويسنده Department of Pathology, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 Serdang,
Selangor, Malaysia. Talib, H. , Yam، M.F. نويسنده , , Jabbarzare، M. نويسنده Department of Human Anatomy, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400
Serdang, Selangor, Malaysia. Jabbarzare, M. , Tie، TH. نويسنده Department of Human Anatomy, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400
Serdang, Selangor, Malaysia. Tie, TH. , Othman، F. نويسنده Department of Human Anatomy, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400
Serdang, Selangor, Malaysia. Othman, F. , Moklas، MAM. نويسنده Department of Human Anatomy, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400
Serdang, Selangor, Malaysia. Moklas, MAM. , Abdullah، WO. نويسنده Department of Medical Microbiology and Parasitology, Faculty of Medicine & Health Sciences, Universiti Putra
Malaysia, 43400 Serdang, Selangor, Malaysia. Abdullah, WO. , Ahmad، Z. نويسنده Department of Pathology, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 Serdang,
Selangor, Malaysia. Ahmad, Z.
Abstract :
Background: Animal models with various combination of host-parasite have long been employed to study malaria pathogenesis. Here, we describe the combination of Plasmodium berghei ANKA infec- tion in inbred ICR mice as a model of cerebral malaria (CM).
Methods: Infection in mice was initiated by intraperitoneal injection of 2 x 107 (0.2ml) parasitized red blood cells (PRBCs).
Results: This model can produce a severe degree of infection presented by the high degree of parasitaemia followed by death 6-7 days post infection. Severe anemia, splenomegaly, hepatomegaly and discolourations of major organs were observed. Histopathological findings revealed several i m- portant features mimicking human CM including, microvascular sequestration of PRBCs in major organs, particularly in the brain, hypertrophy and hyperplasia of the kupffer cells in the liver, pulmo- nary edema and hyaline membrane formation in the lungs and haemorrhages in the kidney’s medulla and cortex. Proinflammatory cytokines TNFα, IFNγ, IL-1, IL-6 and IL-18, and anti-inflammatory cytokine IL-10 were all found to be elevated in the plasma of infected mice.
Conclusion: This model can reproduce many of the important features of CM and therefore can be used as a tool to advance our understanding of the disease pathogenesis.
