Author/Authors :
Zebardast، Nozhat نويسنده Department of Medical Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran , , Haghighi، Ali نويسنده Department of Medical Parasitology & Mycology, Shahid Beheshti University of Medical Sciences, Tehran, , , Yeganeh، Farshid نويسنده Department of Immunology, Shahid Beheshti University of Medical Science, Tehran, Iran. , , Seyyed Tabaei، Seyyed Javad نويسنده Department of Medical Parasitology & Mycology, Shahid Beheshti University of Medical Sciences, Tehran, , , Gharavi، Mohammad Javad نويسنده Department of Parasitology, Iran University of Medical Sciences, Tehran, IR Iran , , Fallahi، Shirzad نويسنده Department of Medical Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran , , Lasjerdi، Zohreh نويسنده Department of Medical Parasitology and Mycology , , Salehi، Nima نويسنده Department of Medical Parasitology and Mycology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, , , Taghipour، Niloofar نويسنده Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, , , Kohansal، Cobra نويسنده Tamin Ejtemaee Hospital, Ahvaz, Iran. Kohansal, Cobra , Naderi، Farideh نويسنده Department of Medical Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran ,
Abstract :
Background: Entamoeba moshkovskii and E. dispar are impossible to differentiate microscopically from the pathogenic species E. histolytica. Multiplex polymerase chain reaction (Multiplex PCR) is a widespread molecular biology technique for amplification of multiple targets in a single PCR experiment.
Methods: For detection and differentiation of the three-microscopy indistinguishable Entamoeba species in human, multiplex PCR assay using different DNA extraction methods was studied. A conserved forward primer was derived from the middle of the small-subunit rRNA gene, and reverse primers were designed from signature sequences specific to each of these three Entamoeba species.
Results: A 166-bp PCR product with E. histolytica DNA, a 580-bp product with E. moshkovskii DNA and a 752-bp product with E. dispar DNA were generated in a single-round and multiplex PCR reaction.
Conclusion: We recommend this PCR assay as an accurate, rapid, and effective diagnostic method for the detection and discrimination of these three Entamoeba species in both routine diagnosis of amoebiasis and epidemiological surveys.
