Author/Authors :
Tabrizizadeh، Mahdi نويسنده Department of Endodontic, Shahid Sadoughi University of Medical Sciences, Yazd, IR Iran , , Rasti، Mojtaba نويسنده Department of Medical Microbiology , School of Medicine , Ahvaz Jundishapur University of Medical Science, Ahvaz, IR Iran Rasti, Mojtaba , Ayatollahi، Fatemeh نويسنده Department of Endodontics, Dental School, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Mossadegh ، Mohammad Hossein نويسنده Department of Pharmacology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Mossadegh , Mohammad Hossein , Zandi، Hengameh نويسنده Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Zandi, Hengameh , Dehghan Marvasti، Farzad نويسنده Student of Dentistry, Dept. of Oral and Maxillofacial Pathology, Yazd Dental School, Shahid Sadughi University of Medical Sciences, Yazd, Iran , , Mousavi، Seyedeh Zohreh نويسنده Department of Speech Therapy, Tehran University of Medical Sciences, Tehran, IR Iran ,
Abstract :
Introduction: Calcium hydroxide (CH) is one of the most common intracanal medications. Corticosteroids (CS) are used in endodontics because of their anti-inflammatory activity. This study aimed to evaluate the antimicrobial effect of CH+betamethasone and CH+saline against Enterococcus faecalis (E. faecalis) using agar diffusion test and measuring the microbial zone of inhibition (ZOI). Methods and Materials: Four plates containing Mueller-Hinton broth and E. faecalis culture media, were prepared. In each plate, 5 holes (5×3 mm) were created and a creamy mixture of CH+betamethasone was inserted into the holes (10 holes for each material). Two holes with ampicillin disks and two empty holes were used as negative and positive controls, respectively. Plates were incubated for 24 h and then the diameter of microbial ZOI was measured. The pH of each mixture was measured by pH meter. Data were analyzed using the Mann-Whitney U test. Results: The mean diameter of ZOI for CH+betamethasone and CH+saline was 3.4 and 3 mm, respectively. The difference was not significant (P=0.143). The pH was 12.5 for CH+saline and 12.3 CH+betamethasone, respectively. Conclusion: The mixture of CH+betamethasone had good antimicrobial effects against E. faecalis. Further studies are needed to confirm the value of this mixture in clinical settings.
